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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical function in endorsing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two extra splice variants were being described but comprehensive nucleotide sequence details is not really still obtainable.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best outcomes are...
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to guarantee the best possible final results are...
Antibodies sure to the blocking peptide no longer bind into the epitope within the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Target Facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which add to your temporal coordination of each and every mitotic event. Cyclin E sorts a posh with and capabilities being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is also degraded as cells development via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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